This was apparently the last horse car used in Denver and survived at least into the 1900s. I do not know what system it may have operated on nor whether it was officially incorporated.
This favorite photo appeared on the cover of Happy Home Chats from Feb. 10, 1936. (Happy Home Chats was a weekly 4-page promotional newletter from Denver's Happy Home Bakers.).
The horse would pull the car and passengers to the top of a gradual hill near today's Englewood. On the downhill trip, the horse would stand on the rear platform and ride down in comfort.